Journal
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
Volume 87, Issue 3, Pages 589-597Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejpb.2014.03.014
Keywords
Multiple emulsions; Squalene; Quil A; Combination vaccine; Diphtheria; Tetanus
Categories
Funding
- Council of Scientific and Industrial Research (CSIR)
- Department of Biotechnology (DBT), New Delhi, India under IYBA [BT/01/IYBA/2009]
Ask authors/readers for more resources
The present work has been envisaged to develop a pH responsive multiple emulsion (W/O/W) adjuvant for successful antigen delivery via oral route. The formulation was prepared by double emulsion technique using squalene as oil phase and a combination of antigens and Quil A as internal aqueous phase. Squalene emulsion (O/W) adjuvants are well accepted for parenteral administration. Instead of using conventional surfactants, Quil A was incorporated in the internal aqueous compartment for the first time. The microscopic analysis confirmed the formation of the W/O/W multiple emulsions with globule diameter ranging from 7 to 12 mu m. Considerably, addition of Quil A resulted in marked improvement in both in vitro stability and immune response. Furthermore, addition of pectin to the external aqueous phase resulted in improved in vitro stability. A formulation with gel like structure was formed when pectin was used as stabilizer and also accounted with a significant increase in viscosity (from 275.3 cP to 330.4 cP) in the simulated gastric fluid, which reverts back to 290.2 cP after exposure to simulated intestirial fluid. Induction of strong mucosal and systemic immune response suggested that the developed formulation could be useful for the delivery of multiple biomolecules via oral route. (C) 2014 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available