Journal
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
Volume 73, Issue 2, Pages 230-238Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejpb.2009.06.015
Keywords
Polymeric micelle; Chemotherapy; Self-assembly; Nanomedicine; Anti-tumor efficacy
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Funding
- FRSM (Belgium)
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Paclitaxel (PTX) is an effective anti-cancer drug currently used to treat a wide variety of cancers. Unfortunately, nonaqueous vehicle containing Cremophor(R) EL is associated with serious clinical side effects. This work aimed to evaluate the ability of polymeric micelles to (i) solubilize PTX without Cremophor(R) EL and to be used as a (ii) safe and (iii) effective delivery system for PTX. Hence, we developed novel self-assembling poly(ethyleneglycol)(750)-block-poly(epsilon-caprolactone-co-trimethylenecarbonate) (PEG-p-(CL-co-TMC) polymeric micelles which form micelles spontaneously in aqueous solution. The solubility of PTX increased up to three orders of magnitude. The PTX-loaded micelles showed a slow release of PTX with no burst effect. The HeLa cells viability assessed by the MTT test was lower for PTX-loaded micelles than for Taxol(R) (IC50 10.6 vs. 17.6 mu g/ml). When solubilized in micelles, M induced apoptosis comparable with Taxol(R). The maximum tolerated doses (MTD) of PTX-loaded micelles and Taxol(R) in mice were 80 mg/kg and 13.5 mg/kg, respectively, after intraperitoneal administration; and 45 mg/kg and 13.5 mg/kg, respectively, after intravenous administration. Similar anti-tumor efficacy of PTX-loaded micelles and Taxol(R) was observed at the dose of 13.5 mg/kg on TLT-tumor-bearing mice, while the body weight loss was only observed in Taxol(R) group. However, as higher dose was tolerated (80 mg/kg-IP), a higher growth delay was induced with PTX-loaded micelles. These results demonstrated that PTX-loaded self-assembling micelles present a similar anti-tumor efficacy as Taxol(R), but significantly reduced the toxicity allowing the increase in the dose for better therapeutic response. (C) 2009 Elsevier B.V. All rights reserved.
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