Journal
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
Volume 2012, Issue 5, Pages 1003-1007Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ejoc.201101476
Keywords
Amines; Amination; Ketones; Transaminases; Biocatalysis
Categories
Funding
- European Union [245144]
- Federal Ministry of Economy, Family and Youth (BMWFJ)
- Federal Ministry of Traffic, Innovation and Technology
- Styrian Business Promotion Agency SFG
- Standortagentur Tirol and ZIT - Technology Agency of the City of Vienna through the COMET
- Evonik Degussa GmbH
- German Federal Ministry of Education and Research (BMBF)
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Two recently identified (S)-selective ?-transaminases (?-TAs) that originate from Paracoccus denitrificans (Strep-PD-?TA, cloned with an N-terminal Strep-tag II) and Pseudomonas fluorescens (PF-?TA) were employed for the asymmetric amination of selected prochiral ketones. The substrates tested were transformed into optically pure amines (>99?% ee) with high conversion (up to >99?%). The ?-TAs led to higher conversion in the absence of dimethyl sulfoxide as a cosolvent than in its presence (15?%, v/v). Additionally, it was shown that a His-tagged recombinant transaminase from Vibrio fluvialis (His-VF-?TA, cloned with an N-terminal His6-tag) showed for a single substrate, ethyl acetoacetate, significantly higher stereoselectivity for the amination compared to the corresponding commercial enzyme preparation (>99 vs. 50?%).
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