Optimization of direct currents to enhance dentine bonding of simplified one-step adhesive

The aim of this study was to investigate the effects of different direct current intensities on dentine bonding effectiveness of Clearfil S-3 Bond and on cell viability of human dental pulp cells (HDPCs). Thirty-five-third molars were sectioned and ground to provide flat surfaces. Clearfil S-3 Bond was applied under different current conditions for 30 s and then resin composite was built up. Specimens were processed for microtensile bond strength (mu TBS) testing and for nanoleakage investigation using scanning electron microscopy. Primary HDPCs isolated from premolars were stimulated with different intensities of electric current for 30 s. Then, cell viability was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Specimens bonded with application of electrical current intensities of 50, 60, 70, and 90 mu A exhibited a significant increase in immediate mu TBS compared with all other groups. Bonded interfaces prepared using electrically assisted current application showed reduced interfacial nanoleakage upon scanning electron microscopy. Electric current application, from 20 to 70 mu A, had no effect on the viability of HDPCs. This study provides further evidence for its future clinical use.