4.1 Article Proceedings Paper

Amelogenin-enamelin association in phosphate-buffered saline

Journal

EUROPEAN JOURNAL OF ORAL SCIENCES
Volume 119, Issue -, Pages 351-356

Publisher

WILEY
DOI: 10.1111/j.1600-0722.2011.00916.x

Keywords

32-kDa enamelin; assembly; circular dichroism; fluorescence spectroscopy; transmission electron microscopy

Funding

  1. NIDCR NIH HHS [R01 DE020099, R01 DE013414, R01 DE-15644, R01 DE-13414, R01 DE015644, R01 DE013414-09] Funding Source: Medline

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The structures and interactions among macromolecules in the enamel extracellular matrix play vital roles in regulating hydroxyapatite crystal nucleation, growth, and maturation. We used dynamic light scattering (DLS), circular dichroism (CD), fluorescence spectroscopy, and transmission electron microscopy (TEM) to investigate the association of amelogenin and the 32-kDa enamelin, at physiological pH 7.4, in phosphate-buffered saline (PBS). The self-assembly behavior of amelogenin (rP148) was altered following addition of the 32-kDa enamelin. Dynamic light scattering revealed a trend for a decrease in aggregate size in the solution following the addition of enamelin to amelogenin. A blue-shift and intensity increase of the ellipticity minima of rP148 in the CD spectra upon the addition of the 32-kDa enamelin, suggest a direct interaction between the two proteins. In the fluorescence spectra, the maximum emission of rP148 was red-shifted from 335 to 341 nm with a marked intensity increase in the presence of enamelin as a result of complexation of the two proteins. In agreement with DLS data, TEM imaging showed that the 32-kDa enamelin dispersed the amelogenin aggregates into oligomeric particles and stabilized them. Our study provides novel insights into understanding the possible cooperation between enamelin and amelogenin in macromolecular co-assembly and in controlling enamel mineral formation.

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