Journal
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
Volume 66, Issue -, Pages 380-387Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.ejmech.2013.05.042
Keywords
Oridonin; Resonance light scattering; DNA; Intracellular target; Binding mode
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Funding
- Natural Science Foundation of Guangdong Province [9151022501000019]
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In this study, a sensitive and reliable assay has been developed to determine the binding mode of oridonin to DNA in vitro using resonance light scattering (RLS) technique. The binding mode of ORI with DNA has been confirmed by measuring the changes of RLS intensity under different pH and ionic strength. It is the ORI that is intercalated into the double helix DNA to destroy its template function and inhibit the synthesis of DNA. Moreover, the RLS assay result was validated by seasoned vitro methods. The proposed RLS assay is not only an unprecedented one for primarily determine the binding mode of oridonin to DNA, but also a simple, sensitive, objective and straightforward method for the detection of ORI. (C) 2013 Elsevier Masson SAS. All rights reserved.
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