Journal
EUROPEAN JOURNAL OF MASS SPECTROMETRY
Volume 17, Issue 4, Pages 395-403Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1255/ejms.1137
Keywords
LC-ESI-MS; pharmacokinetics; Danshen injection; water-soluble components of Danshen
Ask authors/readers for more resources
Salvianolic acid A, salvianolic acid B, danshensu, protocatechuic aldehyde, rosmarinic acid and lithospermic acid are the six major active constituents in Danshen injection. In this study, a rapid, sensitive and specific liquid chromatographic-electrospray ionization-mass spectrometry method for the simultaneous quantitative determination of these compounds in rat plasma was developed. After a single step of liquid-liquid extraction with ethyl acetate, they were eluted by a Hypersil C-18 column (5 mu m, i.d. 4.6 x 200 mm) within 4 min with a mobile phase consisting of acetonitrile and 0.1% formic acid water solution (35:65, v/v). The assay was linear in the concentration range of 0.05-10 mu g mL(-1). Absolute recoveries were above 60%. The precisions and accuracies determined within three consecutive days were within acceptable limits. The method was successfully applied to a pharmacokinetic study in rats after an intravenous administration of Danshen injection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available