Journal
EUROPEAN JOURNAL OF MASS SPECTROMETRY
Volume 16, Issue 2, Pages 221-226Publisher
IM PUBLICATIONS
DOI: 10.1255/ejms.1066
Keywords
diabetic complication; AGE; aminoguanidine; drug repositioning; antibiotic; IC(50)
Funding
- CSIR
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An in vitro insulin glycation assay was developed for screening glycation inhibitors. The assay involves the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for monitoring the formation of glycated insulin. The assay is simple, rapid and amenable for high throughput screening. Using this assay we have discovered a strong anti-glycation activity for the anti-tuberculosis drug rifampicin. These results were compared with bovine serum albumin glucose fluorescence assay. In addition, the IC(50) of rifampicin was lower than that of aminoguanidine, a known anti-glycating agent, suggesting that rifampicin is a more potent glycation inhibitor.
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