Journal
EUROPEAN JOURNAL OF MASS SPECTROMETRY
Volume 14, Issue 3, Pages 153-161Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1255/ejms.917
Keywords
sport; doping; mass spectrometry; SARM; LGD-2226; BMS-564929
Funding
- [Manfred-Donike Institute for Doping Analysis, Cologne]
Ask authors/readers for more resources
Selective androgen receptor modulators (SARMs) have become a major field of clinical research enabling the tissue-selective stimulation of androgen receptors. The treatment of debilitating diseases, osteoporosis and frailty are primary goats and promising results have been obtained from clinical trials. However, the potential for misuse of SARMs in sport is great and drug testing methods based on liquid chromatography were established for different classes including arylpropionamide-, 2-quinolinone- and bicyclic hydantoin-derived compounds. As gas chromatography and mass spectrometry (GC-MS) are still important analytical tools in sports drug testing, a method to determine 2-quinolinone- and bicyclic hydantoin-derived SARMs established. Spiked urine samples were subjected to routine doping control protocols including enzymatic hydrolysis, liquid-Liquid extraction, concentration and derivatisation to trimethylsilylated analogues followed by GC-MS analysis. The method was validated for the items specificity, tower limit of detection (0.2-10 ng mL(-1)), recovery (83-85%), intraday and interday precision (9-15% and 13-18%, respectively), which demonstrates the suitability of conventional GC-MS systems to determine representatives of an emerging class of compounds in doping control specimens.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available