Journal
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
Volume 116, Issue 5, Pages 653-658Publisher
WILEY-BLACKWELL
DOI: 10.1002/ejlt.201300291
Keywords
Alkali metal ion adducts; Cyanopropyl; ESI-MS; Lipid classes; Normal phase HPLC
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Funding
- Lipidor AB, Sweden
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In order to establish a versatile and convenient method for the analysis of lipids, electrospray ionization tandem mass spectrometry (ESI-MS/MS) was applied to a HPLC separation on a cyanopropyl-bonded stationary phase. A binary gradient mobile phase system consisting of hexane, toluene, methanol and a stable electrospray yielding sodium adduct ions could be used to generate specific product ions in MS/MS mode. By applying the LC/ESI-MS/MS method on an egg yolk sample, 29 different molecular species of phosphatidylethanolamines, phosphatidylcholines, and lysophosphatidylcholines could be detected within 25min.
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