Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 44, Issue 10, Pages 2903-2917Publisher
WILEY
DOI: 10.1002/eji.201444612
Keywords
EAE; IFN-gamma; Macrophage; NK cells; T cells
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Funding
- Science Foundation Ireland [11/PI/1036]
- IRC EMBARK Postgraduate Fellowship
- Irish Higher Education Authority (HEA) Programme for Research in Third-Level Institutions (PRTLI)
- Science Foundation Ireland (SFI) [11/PI/1036] Funding Source: Science Foundation Ireland (SFI)
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The role of IFN-gamma in the pathogenesis of autoimmune diseases is controversial. Although Th1 cells can induce experimental autoimmune encephalomyelitis (EAE), IFN-gamma can suppress Th17 cells that are pathogenic in EAE. Here we show that NK cells provide an early source of IFN-gamma during development of EAE. Depletion of NK cells or neutralization of IFN-gamma delayed the onset of EAE and was associated with reduced infiltration of IL-17+ and GM-CSF+ T cells into the CNS. In the passive transfer model, immune cells from myelin oligodendrocyte glycoprotein (MOG)-immunized IFN-gamma(-/-) mice failed to induce EAE, despite producing IL-17 and GM-CSF. The macrophages expressed markers of M2 activation and the T cells had low very late antigen-4 (VLA-4) expression and failed to infiltrate the CNS. Addition of recombinant IFN-gamma to immune cells from the IFN-gamma(-/-) mice activated M1 macrophages and restored VLA-4 expression, migratory, and encephalitogenic activity of T cells. Furthermore, treatment of recipient mice with anti-VLA-4 neutralizing antibody abrogated EAE induced by transfer of T cells from WT mice. Our findings demonstrate IFN-gamma-producing T cells are not required for development of EAE, but NK cell-derived IFN-gamma has a key role in promoting M1 macrophage expansion and VLA-4-mediated migration of encephalitogenic T cells into the CNS.
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