Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 43, Issue 8, Pages 2174-2184Publisher
WILEY
DOI: 10.1002/eji.201343308
Keywords
Co-activator; Histone acetyltransferase activity; IFN; p300; TRIM22
Categories
Funding
- National Natural Science Foundation of China [81072428, 30890141, J1210041]
- Major State Basic Research Development Program of China [2013CB530501]
- Program for Changjiang Scholars and Innovative Research Team in University [PCSIRT-IRT1075]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Natural Science Foundation of the Jiangsu higher education institutions [11KJA180003]
- Qing Lan Project of the Jiangsu higher education institutions
- Shanghai STC grant [09JC1401800]
- Jiangsu Pan-Deng Project [BK2010004]
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Tripartite motif (TRIM) 22 plays an important role in IFN-mediated antiviral activity. We previously demonstrated that IFN regulatory factor-1 (IRF-1) was crucial for constitutive and IFN-induced TRIM22 expression via binding to a special cis-element named 5 extended IFN-stimulating response element. Here, we further investigate the molecular mechanisms of TRIM22 with a focus on the co-activators of IRF-1. Using an in vitro DNA affinity binding assay and an in vivo chromatin immunoprecipitation assay, we found that IFN- stimulation significantly enhanced the binding of p300 and p300/CBP-associated factor, but not other co-activators such as general control nondepressible 5, steroid receptor co-activator-1, and activator of thyroid and retinoic, to the 5 extended IFN-stimulating response element containing TRIM22 promoter region together with IRF-1. Overexpression and knockdown analysis demonstrated that it was p300, but not p300/CBP-associated factor, that functioned as a transcriptional co-activator of IRF-1 in IFN- induction of TRIM22. We further show that p300 contributed to both IFN-- and IRF-1-mediated TRIM22 transcription independent of its histone acetyltransferase activity, however, it was required for the recruitment of RNA polymerase II to TRIM22 promoter region. These data indicate that p300 plays a critical role in IFN--induced TRIM22 expression via recruiting RNA polymerase II to the TRIM22 promoter, and might serve as a bridge between IRF-1 and the basal transcriptional apparatus in TRIM22 induction.
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