Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 42, Issue 11, Pages 2834-2839Publisher
WILEY
DOI: 10.1002/eji.201242999
Keywords
Antigen receptors; Bioinformatics; Next generation sequencing; TCR; VDJ recombination
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Next generation sequencing technologies are revolutionizing the study of immune repertoires. These methods provide a previously unimaginable amount of sequence data, unfortunately accompanied by numerous challenges associated with error correction and interpretation that remain to be solved. For antigen receptors, these challenges will require dedicated solutions beyond those developed for genome sequencing, which may differ depending on the sequencing technology used and the purpose of the experiment. Many investigators are developing such methods, based on different sequencing platforms, but critical details of protocol and performance are proprietary. The field will move forward when these methods are shared and standardized, and when the accuracy, sensitivity and reproducibility of various sequencing, and analytic methods are evaluated using standardized samples in comparative experiments.
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