Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 41, Issue 11, Pages 3312-3322Publisher
WILEY-BLACKWELL
DOI: 10.1002/eji.201041359
Keywords
Adjuvants; Anergy; DCs; alpha-GalCer; Mucosal immunity; NKT
Categories
Funding
- NIH [R21 AI078898, R01 AI079232, P30-AI36211]
- MD Anderson Cancer Center
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Efficient induction of antigen-specific immunity is achieved by delivering multiple doses of vaccine formulated with appropriate adjuvants that can harness the benefits of innate immune mediators. The synthetic glycolipid alpha-galactosylceramide (alpha-GalCer) is a potent activator of NKT cells, a major innate immune mediator cell type effective in inducing maturation of DCs for efficient presentation of co-administered antigens. However, systemic administration of alpha-GalCer results in NKT cell anergy in which the cells are unresponsive to subsequent doses of alpha-GalCer. We show here that alpha-GalCer delivered as an adjuvant by the intranasal route, as opposed to the intravenous route, enables repeated activation of NKT cells and DCs, resulting in efficient induction of cellular immune responses to co-administered antigens. We show evidence that after intranasal delivery, alpha-GalCer is selectively presented by DCs for the activation of NKT cells, not B cells. Furthermore, higher levels of PD-1 expression, a potential marker for functional exhaustion of the NKT cells when alpha-GalCer is delivered by the intravenous route, are not observed after intranasal delivery. These results support a mucosal route of delivery for the utility of alpha-GalCer as an adjuvant for vaccines, which often requires repeated dosing to achieve durable protective immunity.
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