Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 39, Issue 4, Pages 1167-1175Publisher
WILEY
DOI: 10.1002/eji.200839188
Keywords
Collagen; Inhibitory receptor; Leukocyte-associated Ig-like receptor-1 (LAIR-1); Surface interaction and immunoregulation
Categories
Funding
- Medical Research Council
- Medical Research Council [G0400808, G0500845] Funding Source: researchfish
- MRC [G0500845, G0400808] Funding Source: UKRI
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Leukcocyte activity is controlled by numerous interactions between membrane receptors and ligands on the cell surface. These interactions are of low affinity making detection difficult. We developed a sensitive assay that could readily detect extremely weak interactions such as that between CD200 and the activating receptor CD200RLa (K-d > 500 mu M) at the protein level. We used the new technology to screen for interactions of inhibitory receptors for collagens. We confirmed that both human and mouse leukocyte-associated Ig-like receptor-1, and in addition the related inhibitory leukocyte Ig-like receptor subfamily B member 4 (CD85K, Gp49B), bound collagen specifically, whereas other cell surface proteins gave no binding. The monomeric affinities of the interactions were then determined to allow comparison with other leukocyte interactions and indicate conditions when these interactions might lead to inhibitory signals.
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