4.5 Article

Increased expression of the Hutchinson-Gilford progeria syndrome truncated lamin A transcript during cell aging

Journal

EUROPEAN JOURNAL OF HUMAN GENETICS
Volume 17, Issue 7, Pages 928-937

Publisher

NATURE PUBLISHING GROUP
DOI: 10.1038/ejhg.2008.270

Keywords

Hutchinson-Gilford progeria syndrome; laminopathy; lamin A; lamin C; lamin A Delta 150; progerin

Funding

  1. Gun and Bertil Stohne Foundation
  2. Ake Wiberg Foundation
  3. Magnus Bergvall Foundation
  4. Tore Nilsson Foundation
  5. Loo and Hans Osterman Foundation
  6. Torsten and Ragnar Soderberg Foundations
  7. Jeansson Foundations
  8. Swedish Medical Research Council
  9. Swedish Foundation for Strategic Research

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Most cases of the segmental progeroid syndrome, Hutchinson-Gilford progeria syndrome (HGPS), are caused by a de novo dominant mutation within a single codon of the LMNA gene. This mutation leads to the increased usage of an internal splice site that generates an alternative lamin A transcript with an internal deletion of 150 nucleotides, called lamin A Delta 150. The LMNA gene encodes two major proteins of the inner nuclear lamina, lamins A and C, but not much is known about their expression levels. Determination of the overall expression levels of the LMNA gene transcripts is an important step to further the understanding of the HGPS. In this study, we have performed absolute quantification of the lamins A, C and A Delta 150 transcripts in primary dermal fibroblasts from HGPS patients and unaffected age-matched and parent controls. We show that the lamin A Delta 150 transcript is present in unaffected controls but its expression is >160-fold lower than that in samples from HGPS patients. Analysis of transcript expression during in vitro aging shows that although the levels of lamin A and lamin C transcripts remain unchanged, the lamin A Delta 150 transcript increases in late passage cells from HGPS patients and parental controls. This study provides a new method for LMNA transcript analysis and insights into the expression of the LMNA gene in HGPS and normal cells. European Journal of Human Genetics (2009) 17, 928-937; doi:10.1038/ejhg.2008.270; published online 28 January 2009

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