4.5 Article

Strategies for the identification of kinase substrates using analog-sensitive kinases

Journal

EUROPEAN JOURNAL OF CELL BIOLOGY
Volume 89, Issue 2-3, Pages 184-193

Publisher

ELSEVIER GMBH
DOI: 10.1016/j.ejcb.2009.11.024

Keywords

Protein kinase; Analog-sensitive kinase; Substrate identification; Kinase inhibitor; Phosphoproteomics

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Funding

  1. German Research Foundation (DFG) [SFB446/A25]

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Phosphorylation of proteins is a prevalent post-translational modification, which affects intracellular signaling in many ways. About 2% of all eukaryotic genes code for protein kinases catalyzing phosphorylation events. Despite technological advances that have made it possible to identify thousands of phosphorylation sites simultaneously, identification of the substrates of a given kinase remains an exceptionally challenging task. Here, we summarize approaches for substrate identification that make use of genetically engineered 'analog-sensitive' kinases. (C) 2009 Elsevier GmbH. All rights reserved.

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