Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 189, Issue 2, Pages 114-122Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2014.11.010
Keywords
Electron cryo-microscopy; Single-particle analysis; Automated particle picking
Funding
- UK Medical Research Council [MC_UP_A025_1013]
- MRC [MC_UP_A025_1013] Funding Source: UKRI
- Medical Research Council [MC_UP_A025_1013] Funding Source: researchfish
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The selection of particles suitable for high-resolution cryo-EM structure determination from noisy micrographs may represent a tedious and time-consuming step. Here, a semi-automated particle selection procedure is presented that has been implemented within the open-source software RELION. At the heart of the procedure lies a fully CTF-corrected template-based picking algorithm, which is supplemented by a fast sorting algorithm and reference-free 2D class averaging to remove false positives. With only limited user-interaction, the proposed procedure yields results that are comparable to manual particle selection. Together with an improved graphical user interface, these developments further contribute to turning RELION from a stand-alone refinement program into a convenient image processing pipeline for the entire single-particle approach. (C) 2014 The Author. Published by Elsevier Inc.
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