Long-term effects of N fertilization on cropping and growth of olive trees and on N accumulation in soil profile

Two experiments were conducted for 13 years in two olive groves of southern Spain to study the long-term effect of nitrogen (N) fertilization on trees and soil. In the first experiment, 12-year-old 'Picual' olive trees were arranged in a split plot design with method of N application (soil versus a 50% soil:50% foliar combination) as the whole plot factor, and amount of N applied annually (0, 0.12, 0.25, 0.5 or 1.0 kg N tree(-1)) as the subplot factor. In the second experiment, N application to 50-year-old 'Picual' trees was based on the previous season's leaf N concentration. Urea was the source of N in both experiments. During the last 4 years, soil samples were taken at 0-20, 20-40, 40-60, 60-80, and 80-100 cm depth to evaluate the effect of N application on soil eutrophication. Fertilization with N had no significant effects on yield, fruit characteristics, and growth of olive trees for the 13 years of study, even when leaf N concentration increased with the amount of fertilizer N applied. Combining soil and foliar application may reduce the amount of fertilizer N necessary to correct a possible N deficiency because our experiments showed this practice to be more effective in increasing leaf N that applying N only to the soil. Our results question the established deficiency threshold of 1.4% of N in dried leaf because no reduction in yield or growth was observed for lower concentrations. However, leaf N concentration did not drop below 1.2% after 13 years with no N application, probably because of N inputs from rainfall and the mineralization of organic N. Whereas under natural conditions of the non-fertilized treatments NH(4)(+)-N represented the dominant fraction of mineral N in soil, accumulation of high amounts of NO(3)(-)-N in the soil profile occurred in the fertilized plots, which represents a high risk of N leaching from soil. All these results suggest that annual applications of fertilizer N are unnecessary to maintain high productivity and growth in olive. Applying N only when the previous season's leaf analysis indicates that leaf N concentration is below the deficiency threshold, is thus a recommended practice to optimize N fertilization in olive orchards and to reduce N losses by leaching. (C) 2009 Elsevier B.V. All rights reserved.