Journal
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
Volume 235, Issue 6, Pages 1055-1061Publisher
SPRINGER
DOI: 10.1007/s00217-012-1838-0
Keywords
Chromatographic analysis; Pigments; Food matrix; Food analysis; Method validation
Categories
Funding
- Junta de Extremadura of Spain
- FEDER [GR10006]
- Instituto Nacional de Investigacion and Tecnologia Agraria and Alimentaria (INIA)
Ask authors/readers for more resources
The purpose of this paper was to optimize an ultra-high performance liquid chromatography (UHPLC) method for the determination and quantification of carotenoid pigments in food products and compare it with a HPLC method in terms of resolution, selectivity, sensitivity, time and reagent saving. Less analysis times, solvent consumption and better peak resolution were achieved when the UHPLC methodology was used. Ultra-high performance liquid chromatography analysis was performed on Zorbax Eclipse Plus C18 column (2.1 x 50 mm; 1.8 mu m) using a gradient solvent system and diode array detector. A total of seven carotenoids were separated and quantified in < 8.5 min. For all components, the resolution was higher than 1.5 and LOD was below 1.0 mu g mL(-1). To determine the intraday and reproducibility, assays were conducted in 10 continuous days for method validation achieving recuperation rates ranging between 85 and 100 % and no significant differences among the results obtained in same and different days. The proposed methodology was applied for the determination of the carotenoid contents in honeybee pollen samples, pumpkin and nectarine flesh with satisfactory recovery.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available