Journal
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
Volume 231, Issue 3, Pages 467-473Publisher
SPRINGER
DOI: 10.1007/s00217-010-1301-z
Keywords
Carbaryl; Immunochromatographic assay; Colloidal carbon-based immunoassay; Fruit juice
Categories
Funding
- Grant Agency of the Czech Republic [525/07/P273]
- Ministry of Education of the Czech Republic [MSMT 6046137305]
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A simple immunochromatographic assay for sensitive detection of insecticide carbaryl in fruit juices was developed. The test is based on inhibition format on a nitrocellulose membrane strip. The strip was separately coated with rabbit anti-swine antibody (control line) and carbaryl hapten-OVA conjugate (test line). Colour intensity on the test line was possible to recognize visually from that of negative sample within 10 min, with the detection limit of 5 ng mL(-1). All characteristics of the visually evaluated strips were also measured quantitatively, and then the detection limit was 1.5 ng mL(-1). Cross-reactions with other carbamate pesticides were not found (< 1%). No matrix effects were observed when fruit juices (orange, apple, pear, banana) were diluted tenfold times before analyses. The results from immunochromatographical assay were in a good agreement with those obtained by enzyme-linked immunosorbent assay as reference method. All these facts indicate the potential of the immunochromatographic strip for the quality control of fruit juices.
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