Journal
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
Volume 229, Issue 6, Pages 867-874Publisher
SPRINGER
DOI: 10.1007/s00217-009-1115-z
Keywords
Food-borne pathogen; Immunochromatographic test; Nucleic acid lateral flow immunoassay (NALFIA); Listeria monocytogenes; Food; Nucleic acid testing; Nanoparticles
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Funding
- Ministry of Education of Czech Republic [2B08050, MSM 604 613 7305]
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We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with two labelled primer sets, one generic and directed to a specific sequence of the gene encoding 16S rRNA from Listeria spp. and the other specific and directed to a part of the prfA gene encoding the central virulence gene regulator from the food pathogen Listeria monocytogenes (3.5 h). The PCR solution is directly added to the one-step assay device and the appearance of a grey/black line is indicative of the presence of specific amplicons (max 15 min). In all tests performed, the method correctly identified L. monocytogenes and strains of Listeria spp. PCR material of over 20 food samples was tested by NALFIA. The method proved to be useful for the detection of L. monocytogenes in different kinds of food samples.
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