Journal
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
Volume 47, Issue 7, Pages 697-707Publisher
SPRINGER
DOI: 10.1007/s00249-018-1321-3
Keywords
Lignin; Amino-cellulose; Vancomycin; Tetanus toxoid; Glycovaccines
Categories
Funding
- UK Biotechnology and Biological Sciences Research Council [BB/L025477/1]
- BBSRC [BB/L025477/1] Funding Source: UKRI
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The analytical ultracentrifuge (AUC) invented by T. Svedberg has now become an extremely versatile and diverse tool in Biochemistry and Polymer Science for the characterisation of the sizes, shapes and interactions of particles ranging in size from a few nanometres to tens of microns, or in molecular weight, M (molar mass) terms from a few hundred daltons to hundreds of megadaltons. We illustrate this diversity by reviewing recent work on (1) small lignin-like isoeugenols of M similar to 0.4-0.9 kDa for archaeological wood conservation, (2) protein-like association of a functional amino-cellulose M = 3.25 kDa, (3) a small glycopeptide antibiotic (M similar to 1.5 kDa) and its association with a protein involved in antibiotic resistance (M similar to 47 kDa), (4) tetanus toxoid protein TTP (M similar to 150 kDa) and (5) the incorporation of TTP into two huge glycoconjugates considered in glycovaccine development with molecular weight species in a broad distribution appearing to reach 100 MDa. In illustrating the diversity, we will highlight developments in hydrodynamic analysis which have made the AUC such an exciting and important instrument, and point to a potential future development for extending its capability to highly concentrated systems.
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