Journal
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
Volume 39, Issue 2, Pages 337-343Publisher
SPRINGER
DOI: 10.1007/s00249-009-0502-5
Keywords
Biophysics; Single molecule; Magnetic tweezers; Lambda exonuclease; Fragile X syndrome
Categories
Funding
- National Research Council
- NINDS, NIH
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [Z01NS003047, ZIANS003047] Funding Source: NIH RePORTER
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Fragile X syndrome and other trinucleotide diseases are characterized by an elongation of a repeating DNA triplet. The ensemble-averaged lambda exonuclease digestion rate of different substrates, including one with an elongated FMR1 gene containing 120 CGG repeats, was measured using absorption and fluorescence spectroscopy. By use of magnetic tweezers sequence-dependent digestion rates and pausing was measured for individual lambda exonucleases. Within the triplet repeats a lower average and narrower distribution of rates and a higher frequency of pausing was observed.
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