Journal
EUPHYTICA
Volume 172, Issue 3, Pages 313-327Publisher
SPRINGER
DOI: 10.1007/s10681-009-9988-2
Keywords
Molecular markers; Expressed sequence tag-EST; Quantitative trait loci-QTL; Sugarcane
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Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [02/01167-1]
- CTC ( Centro de Tecnologia Canavieira)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [02/01167-1] Funding Source: FAPESP
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Expressed sequence tags derived markers have a great potential to be used in functional map construction and QTL tagging. In the present work, sugarcane genomic probes and expressed sequence tags having homology to genes, mostly involved in carbohydrate metabolism were used in RFLP assays to identify putative QTLs as well as their epistatic interactions for fiber content, cane yield, pol and tones of sugar per hectare, at two crop cycles in a progeny derived from a bi-parental cross of sugarcane elite materials. A hundred and twenty marker trait associations were found, of which 26 at both crop cycle and 32 only at first ratoon cane. A sucrose synthase derived marker was associated with a putative QTL having a high negative effect on cane yield and also with a QTL having a positive effect on Pol at both crop cycles. Fifty digenic epistatic marker interactions were identified for the four traits evaluated. Of these, only two were observed at both crop cycles.
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