Journal
EUKARYOTIC CELL
Volume 7, Issue 7, Pages 1109-1117Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/EC.00036-08
Keywords
-
Categories
Funding
- NIH [AI056070-01A2]
- Wyeth Vaccine Young Investigator Research
- Einstein MMC Center for AIDS Research [NIH NIAID AI51519]
- Interhemispheric Research Training Grant [D43-TW007129]
- Cancer Research Institute
Ask authors/readers for more resources
Monoclonal antibodies (MAbs) to a cell surface histone on Histoplasma capsulatum modify murine infection and decrease the growth of H. capsulatum within macrophages. Without the MAbs, H. capsulatum survives within macrophages by modifying the intraphagosomal environment. In the present study, we aimed to analyze the affects of a MAb on macrophage phagosomes. Using transmission electron and fluorescence microscopy, we showed that phagosome activation and maturation are significantly greater when H. capsulatum yeast are opsonized with MAb. The MAb reduced the ability of the organism to regulate the phagosomal pH. Additionally, increased antigen processing and reduced negative costimulation occur in macrophages that phagocytose yeast cells opsonized with MAb, resulting in more-efficient T-cell activation. The MAb alters the intracellular fate of H. capsulatum by affecting the ability of the fungus to regulate the milieu of the phagosome.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available