Journal
ACS NANO
Volume 9, Issue 5, Pages 5413-5421Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b01181
Keywords
pulmonary surfactant; nanoparticle; aerosol; constrained drop surfactometer; carbon nanotube; graphene nanoplatelet
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Funding
- NSF [CBET-1236596]
- Robert C. Perry Fund of the Hawaii Community Foundation [12ADVC-51367]
- LNM open fund
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1236596] Funding Source: National Science Foundation
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Inhalation of nanoparticles (NP), including lightweight airborne carbonaceous nanomaterials (CNM), poses a direct and systemic health threat to those who handle them. Inhaled NP penetrate deep pulmonary structures in which they first interact with the pulmonary surfactant (PS) lining at the alveolar air water interface. In spite of many research efforts, there is a gap of knowledge between in vitro biophysical study and in vivo inhalation toxicology since all existing biophysical models handle NP PS interactions in the liquid phase. This technical limitation, inherent in current in vitro methodologies, makes it impossible to simulate how airborne NP deposit at the PS film and interact with it. Existing in vitro NP PS studies using liquid-suspended particles have been shown to artificially inflate the no-observed adverse effect level of NP exposure when compared to in vivo inhalation studies and international occupational exposure limits (OELs). Here, we developed an in vitro methodology called the constrained drop surfactometer (CDS) to quantitatively study PS inhibition by airborne CNM. We show that airborne multiwalled carbon nanotubes and graphene nanoplatelets induce a concentration-dependent PS inhibition under physiologically relevant conditions. The CNM aerosol concentrations controlled in the CDS are comparable to those defined in international OELs. Development of the CDS has the potential to advance our understanding of how submicron airborne nanomaterials affect the PS lining of the lung.
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