Journal
JOURNAL OF SEPARATION SCIENCE
Volume 38, Issue 13, Pages 2332-2339Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201500092
Keywords
Capillary electrophoresis; Cisatracurium besylate; Electrochemiluminescence; Laudanosine; Quaternary monoacrylate
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Funding
- key Laboratory of Clinical Laboratory Diagnostics of Ministry of Education [20120615]
- Chongqing Postdoctoral Science Foundation [Xm201313]
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Capillary electrophoresis with electrochemiluminescence detection for the simultaneous analysis of cisatracurium besylate and its degradation products (laudanosine, quaternary monoacrylate) in pharmaceutical preparation was developed and fully validated. The significant parameters that influence capillary electrophoresis separation and electrochemiluminescence detection were optimized. The total analysis time of the analytes was 15 min. The linearities of the method were 0.1 similar to 40.0 mu g/mL for cisatracurium besylate and 0.04 similar to 8.00 mu g/mL for laudanosine, with correlation coefficients (r) of 0.999 and 0.998, respectively. The detection limits (S/N = 3) were 83.0 ng/mL for cisatracurium besylate and 32.0 ng/mL for laudanosine. The intraday relative standard deviations of the analytes were < 3.0%, and the interday relative standard deviations were < 8.0%. The developed method was cost-effective, sensitive, fast, and resource-saving, which was suitable for the ingredient analysis in pharmaceutical preparation.
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