Journal
JOURNAL OF SEPARATION SCIENCE
Volume 38, Issue 4, Pages 663-669Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201401172
Keywords
Amygdalin; High-performance liquid chromatography; Liquid-liquid microextraction; Reversed phase; Sample preparation
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Funding
- Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences
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A novel, simple, and rapid reversed-phase vortex-assisted liquid-liquid microextraction coupled with high-performance liquid chromatography has been introduced for the extraction, clean-up, and preconcentration of amygdalin in oil and kernel samples. In this technique, deionized water was used as the extracting solvent. Unlike the reversed-phase dispersive liquid-liquid microextraction, dispersive solvent was eliminated in the proposed method. Various parameters that affected the extraction efficiency, such as extracting solvent volume and its pH, vortex, and centrifuging times were evaluated and optimized. The calibration curve shows good linearity (r(2) = 0.9955) and precision (RSD < 5.2%) in the range of 0.07-20 mu g/mL. The limit of detection and limit of quantitation were 0.02 and 0.07 mu g/mL, respectively. The recoveries were in the range of 96.0-102.0% with relative standard deviation values ranging from 4.0 to 5.1%. Unlike the conventional extraction methods for plant extracts, no evaporative and re-solubilizing operations were needed in the proposed technique.
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