Journal
JOURNAL OF SEPARATION SCIENCE
Volume 38, Issue 12, Pages 2014-2023Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201500064
Keywords
alpha-Glucosidase inhibitors; High-speed counter-current chromatography; Panax japonicus CA Meyer; Saponins
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Funding
- National Natural Science Foundation of China [31170326, 31370374]
- Jilin Provincial Science and Technology Department [20130413043GH]
- Jilin Provincial Education Department [[2013] 253]
- Natural Science Foundation of Changchun Normal University [[2013] 001]
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A new assay based on ultrafiltration, liquid chromatography and mass spectrometry was developed for the rapid screening and identification of the ligands for alpha-glucosidase from the extract of Panax japonicus. Six saponins were identified as alpha-glucosidase inhibitors. Subsequently, the specific binding ligands, namely, notoginsenoside R-1, ginsenoside Rb-1, chikusetsusaponin V, chikusetsusaponin IV, chikusetsusaponin IVa, and ginsenoside Rd (the purities were 94.18, 95.43, 96.09, 93.26, 94.50, 93.86%, respectively) were separated by counter-current chromatography using two-phase solvent systems composed of tert-butyl methyl ether, acetonitrile, 0.1% aqueous formic acid (3.8:1.0:4.4, v/v/v) and the solvent system composed of methylene chloride, isopropanol, methanol, 0.1% aqueous formic acid (5.8:1.0:6.0:2.2, v/v/v). The results demonstrate that ultrafiltration, liquid chromatography and mass spectrometry combined with high-speed counter-current chromatography might provide not only a powerful tool for screening and isolating alpha-glucosidase inhibitors in complex samples but also a useful platform for discovering bioactive compounds for the prevention and treatment of diabetes mellitus.
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