Journal
JOURNAL OF SEPARATION SCIENCE
Volume 38, Issue 13, Pages 2327-2331Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201500120
Keywords
Capillary zone electrophoresis; Hydrolysis; Monosaccharides; Polysaccharides; Radix Asparagi
Categories
Funding
- Hebei University [SY201415]
- Key Laboratory of Pharmaceutical Quality Control of Hebei Province [Y201501]
Ask authors/readers for more resources
Using orthogonal design, optimized conditions for the hydrolysis of the polysaccharide from Radix Asparagi were determined, as well as its monosaccharide composition. Optimized hydrolysis conditions were a temperature of 100 degrees C in 1.5 M sulfuric acid solution for 5 h. The resulting monosaccharides were derivatized with 1-phenyl-3-methyl-5-pyrazolone, then separated by capillary zone electrophoresis in 40 mM sodium tetraborate buffer (pH 10.1), and detected by ultraviolet absorption at 245 nm. Results indicate that the polysaccharide from Radix Asparagi is composed of xylose, arabinose, glucose, rhamnose, mannose, galactose, glucuronic acid, and galacturonic acid, which differs from published findings. Moreover, xylose, glucuronic acid, and galacturonic acid have not been previously reported in Radix Asparagi polysaccharide. This method is simple, fast, and yields a highly efficient separation. As well, these findings can be applied to quality control of Radix Asparagi and for in-depth study of the biological activity of Radix Asparagi polysaccharide.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available