Journal
JOURNAL OF SEPARATION SCIENCE
Volume 38, Issue 12, Pages 2033-2037Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201401440
Keywords
Electrospray ionization tandem mass spectrometry; Hydrophilic interaction liquid chromatography; Liquid chromatography; Plasma Lipids
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A novel method for the analysis of endogenous lipids and related compounds was developed employing hydrophilic interaction liquid chromatography with electrospray ionization tandem mass spectrometry. A hydrophilic interaction liquid chromatography with carbamoyl stationary phase achieved clear separation of phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, ceramide, and mono-hexsosyl ceramide groups with good peak area repeatability (RSD% < 10) and linearity (R-2 > 0.99). The established method was applied to human plasma assays and a total of 117 endogenous lipids were successfully detected and reproducibly identified. In addition, we investigated the simultaneous detection of small polar metabolites such as amino and organic acids co-existing in the same biological samples processed in a single analytical run with lipids. Our results show that hydrophilic interaction liquid chromatography is a useful tool for human plasma lipidome analysis and offers more comprehensive metabolome coverage.
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