Journal
EPIGENETICS
Volume 7, Issue 1, Pages 29-33Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/epi.7.1.18782
Keywords
DNA methylation; Arabidopsis; de novo; genetic screen; siRNA
Funding
- US National Institutes of Health [GM60398]
- Ministerio de Educacion y Ciencia
- USPHS National Research Service [GM07104]
- Ruth L. Kirschstein National Research Service [GM07185]
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Cytosine DNA methylation is an epigenetic mark frequently associated with silencing of genes and transposons. In Arabidopsis, the establishment of cytosine DNA methylation is performed by DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2). DRM2 is guided to target sequences by small interfering RNAs (siRNAs) in a pathway termed RNA-directed DNA methylation (RdDM). We performed a screen for mutants that affect the establishment of DNA methylation by investigating genes that contain predicted RNA-interacting domains. After transforming FWA into 429 T-DNA insertion lines, we assayed for mutants that exhibited a late-flowering phenotype due to hypomethylated, thus ectopically expressed, copies of FWA. A T-DNA insertion line within the coding region of the spliceosome gene SR45 (sr45-1) flowered late after FWA transformation. Additionally, sr45-1 mutants display defects in the maintenance of DNA methylation. DNA methylation establishment and maintenance defects present in sr45-1 mutants are enhanced in dcl3-1 mutant background, suggesting a synergistic cooperation between SR45 and DICER-LIKE3 (DCL3) in the RdDM pathway.
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