Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 52, Issue 6-7, Pages 331-335Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2013.03.005
Keywords
Geobacillus thermocatenulatus; Biodiesel; Immobilized cells; Lipase; Palm oil; Thermostability
Categories
Funding
- Regional Innovation Creation RD Programs
- Ministry of Economy, Trade and Industry (METI)
- Special Coordination Funds for Promoting Science and Technology, Creation of Innovation Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe), MEXT, Japan
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To develop a robust whole-cell biocatalyst that works well at moderately high temperature (40-50 degrees C) with organic solvents, a thermostable lipase from Geobacillus thermocatenulatus (BTL2) was introduced into an Aspergillus oryzae whole-cell biocatalyst. The lipase-hydrolytic activity of the immobilized A. oryzae (r-BTL) was highest at 50 degrees C and was maintained even after an incubation of 24-h at 60 degrees C. In addition, r-BTL was highly tolerant to 30% (v/v) organic solvents (dimethyl carbonate, ethanol, methanol, 2-propanol or acetone). The attractive characteristics of r-BTL also worked efficiently on palm oil methanolysis, resulting in a nearly 100% conversion at elevated temperature from 40 to 50 degrees C. Moreover, r-BTL catalyzed methanolysis at a high methanol concentration without a significant loss of lipase activity. In particular, when 2 molar equivalents of methanol were added 2 times, a methyl ester content of more than 90% was achieved; the yield was higher than those of conventional whole-cell biocatalyst and commercial Candida antarctica lipase (Novozym 435). On the basis of the results regarding the excellent lipase characteristics and efficient biodiesel production, the developed whole-cell biocatalyst would be a promising biocatalyst in a broad range of applications including biodiesel production. (C) 2013 Elsevier Inc. All rights reserved.
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