Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 51, Issue 2, Pages 113-118Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2012.05.001
Keywords
Diclofenac; Laccase; Nanofiber; Stability; Transformation
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Funding
- Chonbuk National University
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Nanobiocatalysis has received growing attention for use in commercial applications. We investigated the efficiency, stability, and reusability of laccase-poly(lactic-co-glycolic acid) (PLGA) nanofiber for diclofenac transformation. N-H stretching vibrations (3400-3500 cm(-1) and 1560 cm(-1)) in FT-IR spectra confirmed immobilization of laccase on PLGA nanofibers. The relative activity of immobilized laccase was 82% that of free laccase. Immobilized laccase had better storage, pH, and thermal stability than free laccase. The immobilized laccase produced complete diclofenac transformation in three reuse cycles, which was extended to 6 cycles in the presence of syringaldehyde. Results suggest that laccase-PLGA nanofiber may be useful for removing diclofenac from aqueous sources and has potential for other commercial applications. (c) 2012 Elsevier Inc. All rights reserved.
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