Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 51, Issue 1, Pages 47-52Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2012.03.012
Keywords
ZnS quantum dots; Fluorescence intensity; pH probe; Enzyme; Hydrolysis
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Funding
- Medical Science and Technology Development foundation of Guangdong, PR China [B2009190]
- Technology Development foundation of Dongguan, PR China [200910815254]
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Water soluble ZnS quantum clots (QDs) modified by mercaptoacetic acid (MAA) were used to determinate proton concentration in aqueous solutions by fluorescence spectroscopic technique. The results showed that the fluorescence of the water-soluble QDs could be quenched by proton concentration and the fluorescence intensity of the water-soluble QDs decreased linearly as the pH varied from 4.5 to 7.0. Based on this phenomenon, a convenient, rapid and specific method to determine of enzyme reaction kinetics was proposed. The modified ZnS QDs were successfully used as pH probes in monitoring the hydrolysis of glycidyl butyrate catalyzed by porcine pancreatic lipase (PPL). The proposed method was found to improve stability, sensitivity and a monitoring range for determination proton concentration as compared to the already described analytical methods based on p-Nitrophenoxide (PNP). (C) 2012 Elsevier Inc. All rights reserved.
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