Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 47, Issue 3, Pages 64-70Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2010.05.009
Keywords
Nitrile hydratase-amidase cascade system; 3-Cyanopyridine bioconversion; Nicotinamide; Nicotinic acid; Continuous stirred membrane reactor
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Funding
- University of L'Aquila
- ESF [CM0701]
- Ministry of Education of the Czech Republic [0009046]
- Institute of Microbiology [AV0Z50200510]
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The bioconversion of 3-cyanopyridine using the in situ nitrile hydratase-amidase cascade system of resting Microbacterium imperiale CBS 498-74 cells was investigated in an ultrafiltration-membrane reactor, operated in either batch or continuous mode. The effects of operating conditions such as the amount of biocatalyst, substrate concentration, substrate feeding rate, mean residence time, and enzyme-to-substrate ratio, were investigated with the aim of achieving almost 100% substrate conversion and high reactor productivity. As a result, it was found that the NHase-AMase cascade system could be adequately exploited in a continuous reactor configuration. The differing temperature dependence of nitrile hydratase and amidase kinetics enabled the operational parameters to be module d to ensure (i) nitrile hydratase operational stability (at 5 degrees C), and (ii) 100% conversion of 3-cyanopyridine into nicotinic acid, or, alternatively, (iii) enrichment of the effluent stream with the intermediate nicotinamide (up to 80% conversion). It was possible to select operating conditions that allowed long periods of operation (at least 100 h) at a constant flow-rate without enzyme activity loss. (C) 2010 Elsevier Inc. All rights reserved.
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