Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 45, Issue 4, Pages 241-246Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2009.07.006
Keywords
Haematococcus lacustris; Astaxanthin; Stress; Proteomics; MALDI-TOF-MS
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Funding
- Korea Research Foundation [KRF-2006-J00702]
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This study is aimed at identifying the proteins that are up-regulated during astaxanthin accumulation in Haematococcus lacustris. For this H. lacustris cells were cultivated in photobioreactors under normal light irradiance of 40 mu E m(-1) s(-1) for 6 days and then induced to accumulate astaxanthin for 3 days further by exposure to continuous high irradiance of 200 mu E m(-2) s(-1) with fluorescent lamps as light source after the cells reached the stationary phase in a nitrogen-depleted condition. Under this condition, the average astaxanthin content per cell increased from 91 mg/l up to 406 mg/l after 3 days of induction. The proteomics data from a two-dimensional electrophoretic comparison demonstrated that a combination of nitrogen source depletion and I h high light have significantly changed the pattern of protein expression in H. lacustris. A total of 49 protein spots were picked after 1 h of stress induction. They consisted of 13 down-regulated proteins and 36 up-regulated proteins. Fifteen proteins which had highly up-regulated expression were further analyzed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The results will point toward interesting proteins that can be pursued for further analysis of astaxanthin biosynthesis pathway. (C) 2009 Elsevier Inc. All rights reserved.
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