Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 42, Issue 2, Pages 167-172Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2007.09.003
Keywords
pseudomonas fluorescens A2a5; poly(beta-hydroxybutyrate) (PHB); sugarcane liquor; gas chromatography; nuclear magnetic resonance; batch fermentation
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A strain of poly(P-hydroxybutyrate) (PHB)-accumulating bacterium was isolated from the soil in Alaska of USA, identified as Pseudomonas fluorescens, and designated as strain A2a5. The organism grew at temperatures below 30 degrees C, and accumulated a large amount of granules in its cells when it was cultured in the sugarcane liquor medium. The purified sample from cells was determined as PHB by gas chromatographic and nuclear magnetic resonance analysis of polyesters. The cell density of the culture in shaking bottles reached OD600 = 155 with PHB concentration of 31 g l(-1). In 51 bioreactor, a maximum, cell dry weight (CDW) of 32 g l(-1) with a PHB concentration of 22 g l(-1) was obtained, and the PHB content was up to 70% and productivity was 0.23 g l(-1) h(-1). (C) 2007 Elsevier Inc. All rights reserved.
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