Effect of cadmium on the extracellular Na+, K+, and Ca2+ in the gill and small intestine of Goldfish Carassius auratus

In this study, the toxic effect of cadmium on extracellular Na+, K+, and Ca2+ in the gill and small intestine of goldfish Carassius auratus was determined with the technique of ion chromatograph. Two-way ANOVA indicated that the two factors (Cd2+ treatment and time) and the interaction factor had significant effect on the level of Na+, K+, and Ca2+ in the small intestine and gill. 1.0 mg/L Cd2+ significantly increased Ca2+ level in the small intestine, but Ca2+ level in the gill was significantly decreased by 1.0 and 5.0 mg/L Cd2+ at 24, 48, and 72 h. Na+ and K+ level in the small intestine and gill was increased by 1.0 mg/L Cd2+ at three time points, but increased by 5.0 mg/L Cd2+ at a certain different time. In addition, Na+ level was significantly decreased by 5.0 mg/L Cd2+ at 24 or 48h in the small intestine. and gill. The results indicated that Cd2+ played an important role in regulating the level of Na+, K+, and Ca2+ in the small intestine and gill of goldfish C. auratus. A method was constructed to investigate the extracellular Na+, K+ and Ca2+ in the tissues of gold fish with ion chromatography. (C) 2014 Elsevier B.V. All rights reserved.