Journal
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Volume 37, Issue 3, Pages 1236-1242Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.etap.2014.04.020
Keywords
ATTO 647N; Aptamer; Sensor; Lead; SWNT
Funding
- Mashhad University of Medical Sciences
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Lead contamination is a serious environmental problem with toxic effects in human. Here, we developed a simple and sensitive sensing method employing ATTO 647N/aptamer-SWNT ensemble for detection of Pb2+. This method is based on the super quenching capability of single-walled carbon nanotubes (SWNTs), high affinity of the aptamer toward Pb2+ and different propensities of ATTO 647N-aptamer and ATTO 647N-aptamer/Pb2+ complex for adsorption on SWNTs. In the absence of Pb2+, the fluorescence of ATTO 647N-aptamer is efficiently quenched by SWNTs. Upon addition of Pb2+, the aptamer binds to its target, leading to the formation of a G-quadruplex/Pb2+ complex and does not interact with SWNTs and ATTO 647N-aptamer starts fluorescing. This sensor exhibited a high selectivity toward Pb2+ and a limit of detection (LOD) as low as 0.42 nM was obtained. Also this sensor could be applied for detection of Pb2+ ions in tap water and biological sample like serum with high sensitivity. (C) 2014 Elsevier B.V. All rights reserved.
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