Journal
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Volume 38, Issue 2, Pages 595-606Publisher
ELSEVIER
DOI: 10.1016/j.etap.2014.08.009
Keywords
Brominated flame-retardants; Decabrominateddiphenyl ether; Tetrabromobisphenol A; Human serum albumin; Binding interaction
Funding
- Fund for the National Natural Science Foundation of China [21201147]
- Natural Science Foundation of Jiangsu Province [BK2011422, BK2012671]
- Natural Science Foundation of Education Department of Jiangsu Province [12KJA180009, 11KJB150019]
- Jiangsu Fundament of Qilan Project
- 333 Project
- Jiangsu Overseas Research & Training Program for University Prominent Young & Middle-aged Teachers and Presidents
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Decabrominateddiphenyl ether (deca-BDE) and tetrabromobisphenol A (TBBPA) are known as brominated flame-retardants, which are commonly found in the environment. The binding mechanisms of deca-BDE and TBBPA with human serum albumin (HSA) are still unknown. In this report, the interactions of deca-BDE and TBBPA with HSA were investigated using different spectroscopic methods and molecular modeling. The experimental results indicated the formation of complexes between deca-BDE/TBBPA and HSA with different affinity. These interactions affected the secondary structure of HSA. Thermodynamic investigations revealed that hydrophobic forces mainly drove the binding interactions of deca-BDE/TBBPA with HSA. For TBBPA, hydrogen-bonding interactions were also involved in the binding process of TBBPA with HSA. According to the analysis of experimental and theoretical data, we concluded that the binding site of deca-BDE to HSA located in the subdomain IS, while TBBPA was near to subdomain IIA and Trp-214. The binding interactions of deca-BDE and TBBPA with the most prominent carrier protein in the human circulatory system could influence mechanisms of their biochemical processes. Thus, these binding interactions can play central roles in studying the distribution and toxicity mechanisms of brominated flame-retardants. (C) 2014 Elsevier B.V. All rights reserved.
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