Journal
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Volume 36, Issue 3, Pages 1253-1260Publisher
ELSEVIER
DOI: 10.1016/j.etap.2013.10.011
Keywords
Diekol; Ecklonia cava; Adipogenesis; 3T3-L1 preadipocytes; AMPK
Funding
- Technology Commercialization Support Program, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea
Ask authors/readers for more resources
In this study, we assessed the potential inhibitory effect of 5 species of brown seaweeds on adipogenesis the differentiation of 3T3-L1 preadipocytes into mature adipocytes by measuring Oil-Red O staining. The Ecklonia cava extract tested herein evidenced profound adipogenesis inhibitory effect, compared to that exhibited by the other four brown seaweed extracts. Thus, E. cava was selected for isolation of active compounds and finally the three polyphenol compounds of phlorotannins were obtained and their inhibitory effect on adipogenesis was observed. Among the phlorotannins, dieckol exhibited greatest potential adipogenesis inhibition and down-regulated the expression of peroxisome proliferator-activated receptor-gamma (PPAR gamma), CCAAT/enhancer-binding proteins (C/EBP alpha), sterol regulatory element-binding protein 1 (SREBP1) and fatty acid binding protein 4 (FABP4) in a dose-dependent manner. The specific mechanism mediating the effects of dieckol was confirmed by AMP-activated protein kinase (AMPK) activation. These results demonstrate inhibitory effect of dieckol compound on adipogenesis through the activation of the AMPK signal pathway. (c) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available