Journal
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Volume 35, Issue 1, Pages 39-46Publisher
ELSEVIER
DOI: 10.1016/j.etap.2012.10.002
Keywords
Fucoxanthin; Melanoma cell lines (B16F10 cells); Cell arrest; Apoptosis; p27(Kip1); Bcl-xL
Funding
- Marine Bioprocess Research Center of the Marine Bio21 Project
- Ministry of Land, Transport and Maritime, Republic of Korea
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The present study was designed to evaluate the molecular mechanisms of fucoxanthin against melanoma cell lines (B16F10 cells). Fucoxanthin reduced the proliferation of B16F10 cells in a dose-dependent manner accompanied by the induction of cell cycle arrest during the G(0)/G(1) phase and apoptosis. Fucoxanthin-induced G(0)/G(1) arrest was associated with a marked decrease in the protein expressions of phosphorylated-Rb (retinoblastoma protein), cyclin D (1 and 2) and cyclin-dependent kinase (CDK) 4 and up-regulation of the protein levels of p15(INK4B) and p27(KiP1). Fucoxanthin-induced apoptosis was accompanied with the down-regulation of the protein levels of Bcl-xL, an inhibitor of apoptosis proteins (IAPs), resulting in a sequential activation of caspase-9, caspase-3, and PARP. Furthermore, the anti-tumor effect of fucoxanthin was assessed in vivo in Balb/c mice. Intraperitoneal administration of fucoxanthin significantly inhibited the growth of tumor mass in B16F10 cells implanted mice. (C) 2012 Elsevier B.V. All rights reserved.
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