Journal
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Volume 26, Issue 2, Pages 192-198Publisher
ELSEVIER
DOI: 10.1016/j.etap.2008.03.010
Keywords
chlorpyrifos; Gambusia affinis; antioxidant enzymes; oxidative stress; acetylcholinesterase; locomotor behaviour
Funding
- Council of Scientific and Industrial Research (CSIR)
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The recovery effect of chlorpyrifos (CPF) on antioxidant enzymes, locomotor behaviour and the target enzyme acetylcholinesterase (AChE) interaction were studied after exposure to 297 mu g L-1 (LC50 for 96 h) in mosquito fish, Gambusia affinis. Activities of the antioxidant enzymes-superoxide dismutase, catalase, glutathione reductase in viscera, and AChE in brain were inhibited at 96 h of exposure. However, induction in lipid peroxidation was observed. The antioxidant levels were restored to near control by 16-18 days. Similarly, swimming speed and AChE were also recovered but comparatively needs longer period. In vitro AChE study indicated that CPF alters the apparent K. values, resulting in a competitive type of inhibition and the inhibitory constant K-i was found to be 4.57 x 10(-4) M. The results showed that the organophosphate CPF besides its inhibitory effect on target enzyme AChE also inhibits antioxidant enzymes, which can be used as biomarkers in the pesticide-contaminated aquatic streams. (C) 2008 Elsevier B.V. All rights reserved.
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