Journal
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
Volume 32, Issue 8, Pages 1882-1887Publisher
WILEY-BLACKWELL
DOI: 10.1002/etc.2265
Keywords
Zinc; Glyceraldehyde-3-phosphate dehydrogenase; Activation; Kinetics
Categories
Ask authors/readers for more resources
Some aquatic organisms can live in contaminated environment due to their adaptable defense mechanism related to their inducible detoxification and excretion. A recent study showed glyceraldehyde-3-phosphate dehydrogenase (GAPDH) can modulate different cellular activities including transcription activation and detoxification. In the present study, the authors report on experiments to test the GAPDH activity of Chironomus riparius toward heavy metals. Glyceraldehyde-3-phosphate dehydrogenase was isolated and purified from C. riparius. The kinetics of the enzyme was measured. The results showed that GAPDH was inhibited by heavy metals including Co2+, Cu2+, Fe2+, Ni2+, Pb2+, but was activated by zinc ions. The kinetics study of the enzyme showed maximum initial velocity (Vmax) of GAPDH increased by 50%. In addition, the substrate and cofactor affinity increased in the presence of zinc. The GAPDH from C. riparius had maximum activities at pH 8.5 and 37 degrees C. The protein sequence analysis shows that there are 2 additional cysteine and histidine residues in the conserved region of GAPDH from C. riparius, which is believed to play an important role in the interactions with heavy metals. The results suggest that exposure to zinc could modulate GAPDH, which could be related to response of antioxidant defense to other heavy metals. Environ Toxicol Chem 2013;32:1882-1887. (c) 2013 SETAC
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available