Journal
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
Volume 31, Issue 10, Pages 2311-2317Publisher
WILEY
DOI: 10.1002/etc.1950
Keywords
Acetylcholinesterase; Carboxylesterase; Glutathione; Glutathione S-transferase; Organophosphates
Categories
Funding
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT Red 214-2007]
- Universidad Nacional del Comahue, Argentina [PROIN 04-I-004]
Ask authors/readers for more resources
The authors evaluated biomarker responses in caged larvae of the amphibian Rhinella arenarum in water channels during fruit production season and compared them with those elicited by a transient exposure to azinphos methyl (AzM) (0.022?mg/L; 4?h), the main pesticide applied in the Alto Valle region, Patagonia, Argentina, taking into account the maximum environmental concentration detected in superficial water (22.5?mu g/L). The traditional biomarkers of organophosphate exposure, acetylcholinesterase (AChE) and carboxylesterase, were inhibited in tadpoles after one week of exposure in channels potentially receiving pesticide drift, whereas the antioxidant glutathione (GSH) and the detoxifying activity of GSH S-transferase (GST) were induced. In a two-week monitoring study, AChE activity was induced in larvae exposed at the agricultural site, and carboxylesterase showed an inhibition followed by return to control values, suggesting an exposurerecovery episode. Antioxidant glutathione levels were first depleted and then surpassed control levels, whereas GST activity was continuously induced. These responses were mimicked in the laboratory by 2?mg/L AzM-pulse exposure, which notably exceeds the expected environmental concentrations. The results draw attention to the complexity of responses after pesticide exposure, strongly depending on exposure time-concentration and recovery periods, among other possible factors, and support the necessity of the integrated use of biomarkers to assess exposure episodes in agricultural areas. Environ. Toxicol. Chem. 2012; 31: 23112317. (c) 2012 SETAC
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available