ASSESSMENT OF POTENTIAL IMMUNOTOXIC EFFECTS CAUSED BY CYPERMETHRIN, FLUOXETINE, AND THIABENDAZOLE USING HEAT SHOCK PROTEIN 70 AND INTERLEUKIN-1β mRNA EXPRESSION IN THE ANURAN XENOPUS LAEVIS

The current study describes the effect of cypermethrin, fluoxetine, and thiabendazole. at environmentally relevant concentrations, on the expression of heat shock protein 70 (HSP70) and interleukin 1 beta (IL-1 beta). using Xenopus lams larvae as animal model Cytokines and interleukins arc considered good predictors of the immunotoxic potential of xenobiotics Tadpoles at stage 47 (normal tables of X beets) were exposed under static conditions to 0 3 and 30 mu g/L fluoxetine, 0 7 mu g/L thiabendazole. and 0 24 mu g/L cypermethrin The effects were evaluated at 7, 24, and 72 h. and 6 and 9 d Randomly chosen tadpoles were used as genetic material for detection of hsp70 and IL-1 beta mRNA induction through reverse transcription PCR Tadpoles exposed to 30 mu g/L fluoxetine showed mRNA expression of both genes at all exposure times, whereas at 0 3 mu g/L a peak response for hsp70 was observed after 24 h, and the increase in IL-1 beta mRNA was statistically significant with respect to the control 72h after exposure. Thiabendazole induced a high expression of mRNA for both hsp70 and IL-1 beta at all exposure times Cypermethrin increased the hsp70 mRNA levels, with a peak at 24 h. and provoked high expression of IL-1 beta mRNA at all exposure times Considering the relationship between HSP70 and IL-1 beta and their involvement (mainly of IL-1 beta) in immune responses, certain changes observed in their expression could be considered warning indicators of potential immunotoxic effects of these substances on Xenopus Environ Toxicol Chem 2010,29 2536-2543 (C) 2010 SETAC