Journal
ENVIRONMENTAL TECHNOLOGY
Volume 36, Issue 8, Pages 983-991Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09593330.2014.971059
Keywords
methanol dehydrogenase (MDH); methane monooxygenase (MMO); Methylosinus sporium; biogas; methanol; methanotrophs
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Funding
- Korea Ministry of Environment (MOE) as 'Human resource development Project for Waste to Energy'
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Methane in a simulated biogas converting to methanol under aerobic condition was comparatively assessed by inhibiting the activity of methanol dehydrogenase (MDH) of Methylosinus sporium using phosphate, NaCl, NH4Cl or EDTA in their varying concentrations. The highest amount of methane was indistinguishably diverted at the typical conditions regardless of the types of inhibitors: 35 degrees C and pH 7 under a 0.4% (v/v) of biogas, specifically for <40 mM phosphate, 50 mM NaCl, 40 mM NH4Cl or 150 mu M EDTA. The highest level of methanol was obtained for the addition of 40 mM phosphate, 100mM NaCl, 40 mM NH4Cl or 50 mu M EDTA. In other words, 0.71, 0.60, 0.66 and 0.66 mmol methanol was correspondingly generated by the oxidation of 1.3, 0.67, 0.74 and 1.3 mmol methane. It gave a methanol conversion rate of 54.7%, 89.9%, 89.6% and 47.8%, respectively. Among them, the maximum rate of methanol production was observed at 6.25 mu mol/mg h for 100 mM NaCl. Regardless of types or concentrations of inhibitors differently used, methanol production could be nonetheless identically maximized when the MDH activity was limitedly hampered by up to 35%.
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