Journal
ENVIRONMENTAL TECHNOLOGY
Volume 31, Issue 1, Pages 87-95Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09593330903369994
Keywords
Pseudomonas sp.; isolation; ARNr 16S; triacylgycerols; lipase
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A novel, lipid-degrading bacterium (strain AHD-1) was isolated from soil regularly contaminated with washing-machine wastewater in Sfax, Tunisia. When this strain was grown in a medium containing 2% triacylglycerol, the hydrolysis products were found to be diacylglycerols, monoacylglycerols and free fatty acids. This strain was an aerobic, mesophilic, Gram-negative, motile, non-sporulating bacterium, capable of growing optimally at pH 7 and 27 degrees C. The predominant fatty acids were found to be C-16:1 omega 7c (31%), C-16:0(28.1%), C-18:1 omega 7c (16.3%) and C-17:0 (5.8%). Phylogenetic analysis of the 16S rRNA gene showed that this isolate is a new strain belonging to the genus Pseudomonas. Strain AHD-1 was found to be closely related to Pseudomonas azotoformans IAM 1603(T), Pseudomonas gessardii CIP 105469(T) and Pseudomonas libanensis CIP 105460(T) with 99.7%, 99.56% and 99.54% of similarity, respectively.
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